Debajit Das

Plant biotechnologist specializing in CRISPR and plant–insect interactions

debajitdas

Jorhat, Assam, India

Joined April 2026

Summary

Expertise in plant–insect interaction transcriptomics: doctoral work and publications document RNA-seq–based analyses of blackgram responses to bruchid oviposition, identifying defense-related DEGs and pathways. nih+1

Applied genome editing experience: project work and co-authored experiments using CRISPR systems (Cpf1 and Cas9) for editing stress- and disease-related genes in crop species. sciprofiles+2

Broad molecular biology and lab technique proficiency: hands-on skills reported include cloning (conventional and Gateway), Agrobacterium-mediated plant transformation, RNA-seq analysis, PCR/qRT-PCR, protein work (IPTG induction, western blot), ELISA, and microscopy. sciprofiles

Active publication and collaboration record in agricultural biotechnology: multiple peer-reviewed articles and a book chapter on genome editing and stress resilience demonstrate collaborative research across institutes (Assam Agricultural University, CSIR-NEIST and others). google+2

Work

Education

Projects

Writing

Application of CRISPR/Cas-Mediated Genome Editing Techniques in Leguminous Crops

January 1, 2024

Book chapter discussing CRISPR/Cas genome editing applications in legume improvement; Debajit Das listed as a co-author.

link.springer.com

CRISPR/Cas9-Mediated Mutation in XSP10 and SlSAMT Genes Impart Genetic Tolerance to Fusarium Wilt Disease of Tomato (Solanum lycopersicum L.)

January 1, 2023

Study demonstrating CRISPR/Cas9 editing of XSP10 and SlSAMT (single and dual edits) in tomato; dual-gene edits conferred stronger phenotypic tolerance to Fusarium wilt in transient and stable assays.

pubmed.ncbi.nlm.nih.gov

Whole genome sequencing of a novel Bacillus thuringiensis isolated from Assam soil

January 1, 2023

Whole genome sequencing and analysis of a Bt strain from Assam, identification of novel crystal protein-encoding genes and virulence-associated features; co-authored publication includes Debajit Das.

pmc.ncbi.nlm.nih.gov

Bruchid beetle ovipositioning mediated defense responses in black gram pods

January 1, 2021

Generated RNA-seq transcript profiles of developing black gram pods after bruchid oviposition; identified differentially expressed genes and pathways (SA signaling, defensins, secondary metabolites) implicated in oviposition-mediated defense.

pubmed.ncbi.nlm.nih.gov

Genetic, Epigenetic, Genomic and Microbial Approaches to Enhance Salt Tolerance of Plants: A Comprehensive Review

January 1, 2021

Co-authored review covering genetic, genomic, epigenetic and microbial strategies (including genome editing) to enhance plant salt tolerance, and proposing epigenetic approaches such as epiRILs.

mdpi.com